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Repositorio Atenea de la Facultad de Ciencias, UNAM >
Repositorio Ciencias >
FACULTAD DE CIENCIAS >
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Departamento de Biología Celular >
Departamento de Biología Celular >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/11154/1038
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Title: | Functional Diversity of T-Cell Subpopulations in Subacute and Chronic Hypersensitivity Pneumonitis |
Authors: | Barrera, L Mendoza, F Zuniga, J Estrada, A Zamora, AC Melendro, EI Ramirez, R Selman, M Pardo-Cemo, Annie |
Issue Date: | 2008 |
Abstract: | Rationale: Hypersensitivity pneumonitis (HP) exhibits a diverse outcome. Patients with acute/subacute HP usually improve, whereas patients with chronic disease often progress to fibrosis. However, the mechanisms underlying this difference are unknown. Objectives: To examine the T-cell profile from patients with subacute HP and chronic HP. Methods: T cells were obtained by bronchoalveolar lavage from 25 patients with subacute HP, 30 patients with chronic HP, and 8 control subjects. T-cell phenotype and functional profile were evaluated by flow cytometry, cytometric bead array, and immunohistochemistry. Measurements and Main Results: Patients with chronic HP showed higher CD4(+):CD8(+) ratio (median, 3.05 range, 0.3-15 subacute HP: median, 1.3 range, 0.1-10 control: median, 1.3 range, 0.7-2.0 P < 0.01), and a decrease of gamma delta T cells (median, 2.0 range, 0.5-3.4, subacute HP: median, 10 range, 4.8-17 control: median, 15 range, 5-19 P < 0.01). Patients with chronic HP exhibited an increase in the terminally differentiated memory CD4(+) and CD8(+) T-cell subsets compared with patients with subacute HP (P < 0.05). However, memory cells from chronic HIP showed lower IFN-gamma production and decreased cytotoxic activity by CD8(+) T lymphocytes. Chronic HP displayed a Th2-like phenotype with increased CXCR4 expression (median, 6% range, 1.7-36, vs. control subjects: median, 0.7% range, 0.2-1.4 and subacute HP: median, 2.2% range, 0.1-5.3 P < 0.01), and decreased CXCR3 expression (median, 4.3% range, 1.4-25%, vs. subacute HP: median, 37% range, 4.9-78% P < 0.01). Likewise, supernatants from antigen-specific-stimulated cells from chronic HP produced higher levels of IL-4 (80 +/- 63 pg/ml vs. 25 +/- 7 pg/ml P < 0.01), and lower levels of IFN-gamma (3,818 +/- 1671 pg/ml vs. 100 61 pg/ml P < 0.01) compared with subacute HP. Conclusions: Our findings indicate that patients with chronic HP lose effector T-cell function and exhibit skewing toward Th2 activity, which may be implicated in the fibrotic response that characterizes this clinical form. |
URI: | http://hdl.handle.net/11154/1038 |
ISSN: | 1073449X |
Appears in Collections: | Departamento de Biología Celular
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