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http://hdl.handle.net/11154/1691
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Title: | Cigarette smoke exposure potentiates bleomycin-induced lung fibrosis in guinea pigs |
Authors: | Cisneros-Lira, J Gaxiola, M Ramos, C Selman, M Pardo-Cemo, Annie |
Issue Date: | 2003 |
Abstract: | The role of tobacco smoking in the development and outcome of pulmonary fibrosis is uncertain. To approach the effects of cigarette smoke on bleomycin-induced lung fibrosis, we studied five groups of guinea pigs: 1) controls, 2) instilled with bleomycin (B), 3) exposed to tobacco smoke for 6 wk (TS), 4) bleomycin instillation plus tobacco smoke exposure for 6 wk (B + TS), and 5) tobacco smoke exposure for 6 wk and bleomycin after smoking (TS/B). Guinea pigs receiving bleomycin and tobacco smoke exposure exhibited higher fibrotic lesions including a significant increase in the number of positive alpha-smooth muscle actin cells compared with bleomycin alone (B + TS, 3.4 +/- 1.2% TS/ B, 3.7 +/- 1.5% B, 2.3 +/- 1.5% P < 0.01). However, only the TS/ B group reached a significant increase in lung collagen compared with the bleomycin group (TS/B, 3.5 +/- 0.7 B +/- TS, 2.9 +/- 0.4 B, 2.4 +/- 0.2 mg hydroxyproline/lung P < 0.01). Bronchoalveolar lavage (BAL) from TS/ B showed an increased number of eosinophils and higher levels of IL-4 and tissue inhibitor of metalloproteinase-2 (P < 0.01 for all comparisons) and induced a significant increase in fibroblast proliferation (P < 0.05). Importantly, smoke exposure alone induced an increase in BAL neutrophils, matrix metalloproteinase-9, and fibroblast proliferation compared with controls, suggesting that tobacco smoke creates a profibrotic milieu that may contribute to the increased bleomycin-induced fibrosis. |
URI: | http://hdl.handle.net/11154/1691 |
ISSN: | 10400605 |
Appears in Collections: | Departamento de Biología Celular
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