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Repositorio Atenea de la Facultad de Ciencias, UNAM >
Colecciones Ciencias >
Medicina UNAM >
Laboratorio de Inmunoparasitología >
Artículos sobre cisticercosis y T. solium publicados por investigadores mexicanos >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/11154/174322
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| Title: | Actin expression in Taenia solium cysticerci (cestoda): tisular distribution and detection of isoforms |
| Authors: | Ambrosio , JR.
Reynoso-Ducoing, O.
Hernandez-Sanchez, H.
Correa-Pina, D.
González-Malerva, L.
Cruz-Rivera, M.
Flisser, A |
| Issue Date: | 2003 |
| Publisher: | Cell Biology International |
| Citation: | Ambrosio , JR; Reynoso-Ducoing, O; Hernandez-Sanchez, H; Correa-Pina, D; González-Malerva, L; Cruz-Rivera, M; Flisser, A. (2003). Actin expression in Taenia solium cysticerci (cestoda): tisular distribution and detection of isoforms. Cell Biology International, 27(9), 727-733. |
| Abstract: | Identification, localization and partial biochemical characterization of actins expressed in the larval stage of the cestode parasite Taenia solium has been carried out. Frozen tissue sections of cysticerci, the larval stage of this parasite, were reacted with rhodamine-phalloidin, parasite actin was purified by polymerization in the presence of K, mg(�) and ATP actin was analyzed by SDS-PAGE and two-dimensional gel electrophoresis, and immunoblotting of actin was performed in PVDF membranes and with commercial anti-actin monoclonal antibodies. Parasitic tissues showed different fibrous actin fluorescence patterns, which correlated with the expression of isoactins. Purified globular actin had a similar molecular mass to rabbit commercial actin (similar to44 kDa). Actin was resolved into seven isoforms, indicating a family of actin genes. (C) 2003 Published by Elsevier Ltd. |
| URI: | http://hdl.handle.net/11154/99816
10.1016/S1065-6995(03)00142-2
http://hdl.handle.net/11154/174322 |
| ISSN: | 1065-6995 |
| Appears in Collections: | Artículos sobre cisticercosis y T. solium publicados por investigadores mexicanos
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