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http://hdl.handle.net/11154/2169
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Title: | Rhizobium Nod factors induce increases in intracellular free calcium and extracellular calcium influxes in bean root hairs |
Authors: | Cardenas, L Feijo, JA Kunkel, JG Holdaway-Clarke, T Hepler, PK Quinto, C Sánchez, F |
Issue Date: | 1999 |
Abstract: | Application of Nod factors to growing, responsive root hairs of the bean Phaseolus vulgaris induces marked changes in both the intracellular cytosolic free calcium (Ca2+) and in the influx of extracellular [Ca2+]. The intracellular [Ca2+], which has been measured by ratiometric imaging in cells microinjected with fura-2-dextran (70 kDa), elevates within 5 min from approximately 400 nM to 1500 nM in localised zones in the root hair apex. Of particular note is the observation that the elevated regions of [Ca2+] appear to shift position during short time intervals. Increases in and fluctuations of the intracellular [Ca2+] are also observed in the perinuclear region after 10-15 min treatment with Nod factors. The extracellular Ca2+ flux, detected with the non-invasive, calcium specific vibrating electrode, is inwardly directed and also increases quickly in response to Nod factors from 13 pmol cm(-2) s(-1) to 28 pmol cm(-2) s(-1). Chitin-oligomers, which are structurally similar but biologically inactive when compared to the active Nod factors, fail to elicit changes in either intracellular or extracellular Ca2+. The similar timing and location of the intracellular elevations and the increased extracellular influx provide support for the idea that Ca2+ participates in secretion and cell wall remodelling, which occur in anticipation of root hair deformation and curling. |
URI: | http://hdl.handle.net/11154/2169 |
ISSN: | 0960-7412 |
Appears in Collections: | Ciencias
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