|
Repositorio Atenea de la Facultad de Ciencias, UNAM >
Repositorio Ciencias >
FACULTAD DE CIENCIAS >
Biología >
Departamento de Biología Celular >
Departamento de Biología Celular >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/11154/2580
|
Title: | Surfactant components modulate fibroblast apoptosis and type I collagen and collagenase-1 expression |
Authors: | De Lara, LV Becerril, C Montano, M Ramos, C Maldonado, V Melendez, J Phelps, DS Selman, M Pardo-Cemo, Annie |
Issue Date: | 2000 |
Abstract: | During lung injury, fibroblasts migrate into the alveolar spaces where they can be exposed to pulmonary surfactant. We examined the effects of Survanta and surfactant protein A (SP-A) on fibroblast growth and apoptosis and on type I collagen, collagenase-1, and tissue inhibitor of metalloproteinase (TIMP)-1 expression. Lung fibroblasts were treated with 100, 500, and 1,000 mg/ml of Survanta 10, 50, and 100 mg/ml of SP-A and 500 mg/ml of Survanta plus 50 mg/ml of SP-A. Growth rate was evaluated by a formazan-based chromogenic assay, apoptosis was evaluated by DNA end labeling and ELISA, and collagen, collagenase-1, and TIMP-1 were evaluated by Northern blotting. Survanta provoked fibroblast apoptosis, induced collagenase-1 expression, and decreased type I collagen affecting mRNA stability similar to 10-fold as assessed with the use of actinomycin D. Collagen synthesis and collagenase activity paralleled the gene expression results. SP-A increased collagen expression similar to2-fold and had no effect on collagenase-1, TIMP-1, or growth rate. When fibroblasts were exposed to a combination of Survanta plus SP-A, the effects of Survanta were partially reversed. These findings suggest that surfactant lipids may protect against intraluminal fibrogenesis by inducing fibroblast apoptosis and decreasing collagen accumulation. |
URI: | http://hdl.handle.net/11154/2580 |
ISSN: | 10400605 |
Appears in Collections: | Departamento de Biología Celular
|
Files in This Item:
There are no files associated with this item.
|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
|