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http://hdl.handle.net/11154/2604
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Title: | Cloning and expression of guinea pig TIMP-2. Expression in normal and hyperoxic lung injury |
Authors: | Melendez, J Maldonado, V Bingle, CD Selman, M Pardo-Cemo, Annie |
Issue Date: | 2000 |
Abstract: | Tissue inhibitors of metalloproteinases (TIMPs) play a key regulatory role in extracellular matrix remodeling. By screening a lung library with a human TIMP-2 cDNA probe, we have isolated the cDNA corresponding to guinea pig TIMP-2. The 3.5-kb cDNA presents an open reading frame that predicts a protein of 220 amino acids showing 97.2, 96.8, 97.2, and 77.3% overall identity with human, mouse, rat, and chicken TIMP-2, respectively. Guinea pig TIMP-2 cDNA was expressed in CHO-K1 cells, showing a protein with the expected molecular weight and activity. Northern blot analysis revealed TIMP-2 expression in brain, kidney, intestine, spleen, heart, and lung. Transforming growth factor-beta down-regulated TIMP-2 mRNA in guinea pig lung fibroblasts, whereas a variety of other stimuli showed no effect. In normal and hyperoxia-exposed lungs, TIMP-2 mRNA was mainly localized in alveolar macrophages and epithelial cells. No quantitative differences were found by Northern blot. These results confirm that TIMP-2 is highly conserved in mammals and largely expressed in lungs. |
URI: | http://hdl.handle.net/11154/2604 |
ISSN: | 1040-0605 |
Appears in Collections: | Departamento de Biología Celular
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