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http://hdl.handle.net/11154/2679
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Title: | The retina of crayfish Procambarus clarkii during development shows serotonin and tryptophan hydroxylase-iike immunoreactivity daily changes |
Authors: | Escamilla Chimal, EG Aguilar Morales, M Romero Diaz, VJ Fanjul Moles, ML García Rivera, CC |
Issue Date: | 1998 |
Abstract: | The aim of this work was to investigate possible changes in serotonin (5-HT) and tryptophan hydroxylase (TPH)-like immunoreactivity related to time of day in Procambarus clarkii retina during the first developmental stages. Forty-five animals from postembryonic instars (PO1, PO2) to juvenile stage were kept under LD 12:12 cycles. All animals were anesthetized and decapitated at three times of day, 08:00, 15:00 and 20:00 h. Isolated eyestalks were processed by immunohistochemical methods. The 5-HT-like immunoreactive area of retina was measured using computer-based image analysis. Results indicated 5-HT-like immunoreactive differences among the three crayfish instars studied. In PO1 animals, ANOVA revealed no significant differences in 5-HT-like immunoreactivity in the retina at different times of day. PO2 instars as well as juvenile instars, showed statistically significant retinal 5-HT-like immunoreactivity changes related to time of day. Preliminary results indicated that TPH-like immunoreactivity was located only in the tapetal and retinal cells, and it was related to time of day. These changes suggest a diurnal cyclic regulation in the synthesis of 5-HT in the retina. |
URI: | http://hdl.handle.net/11154/2679 |
ISSN: | 0929-1016 |
Appears in Collections: | Departamento de Biología Comparada
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