Circadian oscillations of RPCH gene expression in the eyestalk of the crayfish Cherax quadricarinatus

Abstract

The RPCH and beta-actin cDNAs from the crayfish Cherax quadricarinatus were amplified, cloned and sequenced. The primary structure sequences of these cDNAs were compared to other members of the AKH/RPCH family. Fluctuations in the amount of the C quadricarinatus RPCH and beta-actin mRNAs, as cDNAs, were quantified every 3 h by RT-PCR. Single cosinor analysis supports the notion of beta-actin and RPCH mRNA circadian behavior in animals subjected to 12 h: 12 h light/dark regimes. In constant darkness RPCH mRNA concentration changes to ultradian cycles. (c) 2005 Elsevier Inc. All rights reserved.