Olvera, A; Ramos-Cerrillo, B; Estevez, J; Clement, H; de Roodt, A; Paniagua-Solis, J; Vazquez, H; Zavaleta, A; Arruz, MS; Stock, RP; Alagon, A
(2006)
We report the cloning of sphingomyelinase D (SMD) cDNA from Loxosceles reclusa, Loxosceles boneti and Loxosceles laeta into bacterial expression systems, as well as optimization of expression conditions so as to obtain ...