dc.contributor.author |
Barrera, L |
|
dc.contributor.author |
Mendoza, F |
|
dc.contributor.author |
Zuniga, J |
|
dc.contributor.author |
Estrada, A |
|
dc.contributor.author |
Zamora, AC |
|
dc.contributor.author |
Melendro, EI |
|
dc.contributor.author |
Ramirez, R |
|
dc.contributor.author |
Selman, M |
|
dc.contributor.author |
Pardo-Cemo, Annie |
|
dc.date.accessioned |
2011-01-22T10:27:07Z |
|
dc.date.available |
2011-01-22T10:27:07Z |
|
dc.date.issued |
2008 |
|
dc.identifier.issn |
1073449X |
|
dc.identifier.uri |
http://hdl.handle.net/11154/1038 |
|
dc.description.abstract |
Rationale: Hypersensitivity pneumonitis (HP) exhibits a diverse outcome. Patients with acute/subacute HP usually improve, whereas patients with chronic disease often progress to fibrosis. However, the mechanisms underlying this difference are unknown. Objectives: To examine the T-cell profile from patients with subacute HP and chronic HP. Methods: T cells were obtained by bronchoalveolar lavage from 25 patients with subacute HP, 30 patients with chronic HP, and 8 control subjects. T-cell phenotype and functional profile were evaluated by flow cytometry, cytometric bead array, and immunohistochemistry. Measurements and Main Results: Patients with chronic HP showed higher CD4(+):CD8(+) ratio (median, 3.05 |
en_US |
dc.description.abstract |
range, 0.3-15 |
en_US |
dc.description.abstract |
subacute HP: median, 1.3 |
en_US |
dc.description.abstract |
range, 0.1-10 |
en_US |
dc.description.abstract |
control: median, 1.3 |
en_US |
dc.description.abstract |
range, 0.7-2.0 |
en_US |
dc.description.abstract |
P < 0.01), and a decrease of gamma delta T cells (median, 2.0 |
en_US |
dc.description.abstract |
range, 0.5-3.4, subacute HP: median, 10 |
en_US |
dc.description.abstract |
range, 4.8-17 |
en_US |
dc.description.abstract |
control: median, 15 |
en_US |
dc.description.abstract |
range, 5-19 |
en_US |
dc.description.abstract |
P < 0.01). Patients with chronic HP exhibited an increase in the terminally differentiated memory CD4(+) and CD8(+) T-cell subsets compared with patients with subacute HP (P < 0.05). However, memory cells from chronic HIP showed lower IFN-gamma production and decreased cytotoxic activity by CD8(+) T lymphocytes. Chronic HP displayed a Th2-like phenotype with increased CXCR4 expression (median, 6% |
en_US |
dc.description.abstract |
range, 1.7-36, vs. control subjects: median, 0.7% |
en_US |
dc.description.abstract |
range, 0.2-1.4 |
en_US |
dc.description.abstract |
and subacute HP: median, 2.2% |
en_US |
dc.description.abstract |
range, 0.1-5.3 |
en_US |
dc.description.abstract |
P < 0.01), and decreased CXCR3 expression (median, 4.3% |
en_US |
dc.description.abstract |
range, 1.4-25%, vs. subacute HP: median, 37% |
en_US |
dc.description.abstract |
range, 4.9-78% |
en_US |
dc.description.abstract |
P < 0.01). Likewise, supernatants from antigen-specific-stimulated cells from chronic HP produced higher levels of IL-4 (80 +/- 63 pg/ml vs. 25 +/- 7 pg/ml |
en_US |
dc.description.abstract |
P < 0.01), and lower levels of IFN-gamma (3,818 +/- 1671 pg/ml vs. 100 61 pg/ml |
en_US |
dc.description.abstract |
P < 0.01) compared with subacute HP. Conclusions: Our findings indicate that patients with chronic HP lose effector T-cell function and exhibit skewing toward Th2 activity, which may be implicated in the fibrotic response that characterizes this clinical form. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
Functional Diversity of T-Cell Subpopulations in Subacute and Chronic Hypersensitivity Pneumonitis |
|
dc.type |
Artículo de investigación |
en_US |
dc.identifier.idprometeo |
1012 |
|
dc.source.novolpages |
177(1):44-55 |
|
dc.subject.wos |
Critical Care Medicine |
|
dc.subject.wos |
Respiratory System |
|
dc.description.index |
WoS: SCI, SSCI o AHCI |
|
dc.subject.keywords |
allergic alveolitis |
|
dc.subject.keywords |
cytotoxic |
|
dc.subject.keywords |
hypersensitivity pneumonitis |
|
dc.subject.keywords |
T cells |
|
dc.subject.keywords |
Th1/Th2 cells |
|
dc.relation.journal |
American Journal of Respiratory and Critical Care Medicine |
|
dc.description.Departamento |
Departamento de Biología Comparada |
|