Local and Circulating Microchimerism is Associated with Hypersensitivity Pneumonitis

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Show simple item record Bustos, ML Frias, S Ramos, S Estrada, A Arreola, JL Mendoza, F Gaxiola, M Salcedo, M Selman, M Pardo-Cemo, Annie 2011-01-22T10:27:08Z 2011-01-22T10:27:08Z 2007
dc.identifier.issn 1073449X
dc.description.abstract Rationale: Hypersensitivity pneumonitis (HP) is a lymphocytic alveolitis provoked by exposure to a variety of antigens. However, the disease occurs in only a subset of exposed individuals, suggesting that additional factors may be involved. Microchimerism has been implicated in the pathogenesis of autoimmune diseases, especially in those showing increased incidence after childbearing age. Objectives: To evaluate the presence of circulating and local microchimeric cells in female patients with HP. Methods: Male microchimerism was examined in 103 patients with HP, 30 with idiopathic pulmonary fibrosis (IPF), and 43 healthy women. All of them had given birth to at least one son, with no twin siblings, blood transfusions, or transplants. Microchimerism was examined by dot blot hybridization (peripheral blood), and by fluorescence in situ hybridization in bronchoalveolar lavage cells and lungs. Measurements and Main Results: Blood microchimerism was found in 33% of the patients with HP in comparison with 10% in those with IPF (p = 0.019) and 16% in healthy women (p = 0.045). Patients with HP with microchimerism showed a significant reduction of diffusing capacity of carbon monoxide (DLCO en_US
dc.description.abstract 53.5 +/- 11.9% vs. 65.2 +/- 19.7% en_US
dc.description.abstract p = 0.02) compared with patients with HP without microchimerism. In bronchoalveolar lavage cells, microchimerism was detected in 9 of 14 patients with HP compared with 2 of 10 patients with IPF (p 0.047). Cell sorting revealed that microchimeric cells were either macrophages or CD4(+) or CD8(+) T cells. Male microchimeric cells were also found in the five HP lungs examined by fluorescence in situ hybridization. Conclusions: Our findings (1) demonstrate that patients with HP exhibit increased frequency of fetal microchimerism, (2) confirm the multilineage capacity of microchimeric cells, and (3) suggest that microchimeric cells may increase the severity of the disease. en_US
dc.language.iso en en_US
dc.title Local and Circulating Microchimerism is Associated with Hypersensitivity Pneumonitis
dc.type Artículo de investigación en_US
dc.identifier.idprometeo 1144
dc.identifier.doi 10.1164/rccm.200608-1129OC
dc.source.novolpages 176(1):90-95
dc.subject.wos Critical Care Medicine
dc.subject.wos Respiratory System
dc.description.index WoS: SCI, SSCI o AHCI
dc.subject.keywords allergic extrinsic alveolitis
dc.subject.keywords hypersensitivity pneumonitis
dc.subject.keywords microchimerism
dc.relation.journal American Journal of Respiratory and Critical Care Medicine
dc.description.Departamento Departamento de Biología Comparada

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