Karyotype analysis of Eucinostomus argenteus, E-gula, E-harengulus, and Eugerres plumieri (Teleostei, Gerreidae) from Florida and Puerto Rico

Abstract

The karyotypes of four gerreids of the western Atlantic Ocean are documented. A diploid chromosome complement of 48 telocentric chromosomes was found in the four species (2N = 48t, fundamental number FN = 48). No differences were detected either in the number of chromosomes of the standard karyotype, in their karyotype size, or between the karyotypes derived from male or female specimens of any of the species. Chromosome length decreased progressively and slightly from pair 1 to pair 24. The Ag - NOR karyotypes of E. argenteus and E. harengulus were characterized by the position of the nucleolar organizer regions next to the centromere in chromosome pair 1, whereas in E. gula and E. plumieri Ag - NORs were detected in pair 4. The other 46 chromosomes showed a light staining of the centromere with no terminal or intermediate heterochromatic blocks. All Eucinostomus species showed Ag - NORs of similar size, while Eugerres plumieri showed Ag - NORs 10 - 20% larger than Eucinostomus species. A combination of size and position of the Ag - NORs identified E. gula, while size alone identified E. plumieri. However, the ancestral state for size and position of Ag - NORs could not be established. There was no differential staining of the chromosomes by G-banding. The karyotype of the gerreids appears similar to the hypothetical ancestral karyotype of fish. The phylogenetic relationships among these species could not be established because of the lack of chromosome G-bands. Most likely this indicates a homogeneous distribution of GC nucleotides in the chromosomes.