dc.contributor.advisor |
|
|
dc.contributor.author |
Cisneros-Lira, J |
|
dc.contributor.author |
Gaxiola, M |
|
dc.contributor.author |
Ramos, C |
|
dc.contributor.author |
Selman, M |
|
dc.contributor.author |
Pardo-Cemo, Annie |
|
dc.date.accessioned |
2011-01-22T10:26:45Z |
|
dc.date.available |
2011-01-22T10:26:45Z |
|
dc.date.issued |
2003 |
|
dc.identifier.issn |
10400605 |
|
dc.identifier.uri |
http://hdl.handle.net/11154/1691 |
|
dc.description.abstract |
The role of tobacco smoking in the development and outcome of pulmonary fibrosis is uncertain. To approach the effects of cigarette smoke on bleomycin-induced lung fibrosis, we studied five groups of guinea pigs: 1) controls, 2) instilled with bleomycin (B), 3) exposed to tobacco smoke for 6 wk (TS), 4) bleomycin instillation plus tobacco smoke exposure for 6 wk (B + TS), and 5) tobacco smoke exposure for 6 wk and bleomycin after smoking (TS/B). Guinea pigs receiving bleomycin and tobacco smoke exposure exhibited higher fibrotic lesions including a significant increase in the number of positive alpha-smooth muscle actin cells compared with bleomycin alone (B + TS, 3.4 +/- 1.2% |
en_US |
dc.description.abstract |
TS/ B, 3.7 +/- 1.5% |
en_US |
dc.description.abstract |
B, 2.3 +/- 1.5% |
en_US |
dc.description.abstract |
P < 0.01). However, only the TS/ B group reached a significant increase in lung collagen compared with the bleomycin group (TS/B, 3.5 +/- 0.7 |
en_US |
dc.description.abstract |
B +/- TS, 2.9 +/- 0.4 |
en_US |
dc.description.abstract |
B, 2.4 +/- 0.2 mg hydroxyproline/lung |
en_US |
dc.description.abstract |
P < 0.01). Bronchoalveolar lavage (BAL) from TS/ B showed an increased number of eosinophils and higher levels of IL-4 and tissue inhibitor of metalloproteinase-2 (P < 0.01 for all comparisons) and induced a significant increase in fibroblast proliferation (P < 0.05). Importantly, smoke exposure alone induced an increase in BAL neutrophils, matrix metalloproteinase-9, and fibroblast proliferation compared with controls, suggesting that tobacco smoke creates a profibrotic milieu that may contribute to the increased bleomycin-induced fibrosis. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
Cigarette smoke exposure potentiates bleomycin-induced lung fibrosis in guinea pigs |
|
dc.type |
Artículo de investigación |
en_US |
dc.identifier.idprometeo |
1959 |
|
dc.identifier.doi |
10.1152/ajplung.00074.2003 |
|
dc.source.novolpages |
285(4) |
|
dc.subject.wos |
Physiology |
|
dc.subject.wos |
Respiratory System |
|
dc.description.index |
WoS: SCI, SSCI o AHCI |
|
dc.subject.keywords |
matrix metalloproteinase-9 |
|
dc.subject.keywords |
fibroblast proliferation |
|
dc.subject.keywords |
interleukin-4 |
|
dc.subject.keywords |
tissue inhibitor of metalloproteinase-2 |
|
dc.subject.keywords |
pulmonary fibrosis |
|
dc.relation.journal |
American Journal of Physiology-Lung Cellular and Molecular Physiology |
|
dc.description.Departamento |
Departamento de Biología Comparada |
|