Standardization of an experimental model of human taeniosis for oral vaccination

DSpace/Manakin Repository

Show simple item record Leon-Cabrera, Sonia Cruz-Rivera, Mayra Mendlovic, Fela Avila -Ramirez, Guillermina Cesar Carrero, Julio Pedro Laclette, Juan Flisser, Ana 2011-12-07T13:47:24Z 2014-01-28T15:16:54Z 2011-12-07T13:47:24Z 2014-01-28T15:16:54Z 2009
dc.identifier.citation Leon-Cabrera, Sonia; Cruz-Rivera, Mayra; Mendlovic, Fela; Avila -Ramirez, Guillermina; Cesar Carrero, Julio; Pedro Laclette, Juan; Flisser, Ana. (2009). Standardization of an experimental model of human taeniosis for oral vaccination. Methods, 49(4) 346-350.,
dc.identifier.issn 1046-2023
dc.identifier.uri 10.1016/j.ymeth.2009.07.007
dc.description.abstract Neurocysticercosis in humans is caused by the tapeworm Taenia solium and generates substantial morbidity in Latin America, Africa and Asia. The life cycle of T solium includes pigs as intermediate hosts and human beings as definitive hosts. Tapeworm carriers are the main risk factor for acquiring cysticercosis in the household, thus prevention and control programs are being developed. Infected people have no symptoms, therefore are difficult to identify and treat, thus vaccination against the adult tapeworm is an alternative control measure. Since the infection occurs naturally only in human beings, experimental models have been standardized. Hamsters are believed to be good models to study the infection but they have not been properly evaluated for vaccination. Since taeniosis is gained by ingesting pork meat with cysticerci, oral vaccination was evaluated, and given that intestinal immunity is enhanced with adjuvants, cholera toxin was used, because it is one of the most potent adjuvants, in view of the fact that it increases epithelium permeability enhancing entrance of the co-administered unrelated antigens. Recombinant functional T solium calreticulin was employed for the standardization of the methodology and the evaluation of oral vaccination. Protection was associated with the type of cysticerci and the age of the hamsters used. When reddish bigger parasites were orally introduced in hamsters as challenge, protection was around 40%, while when yellowish small parasites were used, protection increased to 100%, suggesting that the characteristics of cysticerci are determinant. Protection was gained in 9 month old hamsters, but not in 3 month old animals. (c) 2009 Elsevier Inc. All rights reserved.
dc.language.iso en
dc.publisher Methods
dc.subject.classification 1022
dc.title Standardization of an experimental model of human taeniosis for oral vaccination
dc.type Article
dc.relation.index WoS

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account