Further ultrastructural characterization of the intranuclear ring-shaped bodies of the plant Lacandonia schismatica

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Show simple item record Agredano-Moreno, LT Segura-Valdez, ML Ubaldo, E Ramos, CH Martínez, E Jiménez-García, LF González-Jimenez, MA 2011-01-22T10:26:55Z 2011-01-22T10:26:55Z 2001
dc.identifier.issn 10478477
dc.description.abstract Ring-shaped bodies are found in the nucleus of Lacandonia schismatica, a rare plant with the sexual organs inverted. They are 0.5-mum-diameter structures that present an electron-dense external ring surrounding a central core. Ultrastructural studies indicate that these bodies contain RNA. The external ring is labeled with antibodies against small nuclear ribonucleoproteins, suggesting that they may be involved in pre-mRNA metabolism. In the present work we further characterized these intranuclear ring-shaped structures by serial-sectioning analysis. Moreover, we tested the presence of additional molecular elements related to pre-mRNA metabolism, such as SR proteins and poly(A)(+) RNA, using immunoelectron microscopy and ultrastructural in situ hybridization. Our results show that these nuclear bodies are spherical. They contain SR proteins involved in splicing and postsplicing events and little to no poly(A)(+) RNA. We also found similar nuclear bodies in other plant and animal species. Therefore, ring-shaped bodies in L. schismatica are spherical, highly compartmentalized nuclear structures that may be involved in pre-mRNA metabolism. (C) 2001 Elsevier Science (USA). en_US
dc.language.iso en en_US
dc.title Further ultrastructural characterization of the intranuclear ring-shaped bodies of the plant Lacandonia schismatica
dc.type Artículo de investigación en_US
dc.identifier.idprometeo 2242
dc.identifier.doi 10.1006/jsbi.2001.4416
dc.source.novolpages 136(1):1-6
dc.subject.wos Biochemistry & Molecular Biology
dc.subject.wos Biophysics
dc.subject.wos Cell Biology
dc.description.index WoS: SCI, SSCI o AHCI
dc.subject.keywords cell nucleus
dc.subject.keywords Lacandonia schismatica
dc.subject.keywords nuclear bodies
dc.subject.keywords ring-shaped bodies
dc.relation.journal Journal of Structural Biology
dc.description.Departamento Departamento de Biología Comparada

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