daily and circadian expression of cryptochrome during the ontogeny of crayfish

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Show simple item record Escamilla-Chimal, Elsa Fanjul, ML 2011-01-22T10:25:58Z 2011-01-22T10:25:58Z 2011-01-22T10:25:58Z 2011-01-22T10:25:58Z 2008
dc.identifier.issn 1095-6433
dc.description.abstract Cryptochromes (CRY) are proteins with a dual role in the circadian function of different animals, participating in phototransduction and light signaling to the clock and as a transcriptional repressor that provides negative feedback in the clock feedback loop. Here we characterize functional expression of CRY as a marker of the functionality of the circadian pacemaker of crayfish (Procambarus clarkii) throughout postembryonic development. Using different experimental light protocols and by means of immunofluorescence and biochemical methods, we report that, as in the adult, in young crayfish from the first embryonic stage CRY is present in cells adjacent to the eyestalk hemiellipsoidal body and the anterior margin of the brain protocerebrum. In the brain, CRY cycles after 72 h darkness, entraining to LD cycles. Meanwhile, as in the adult eye, in juveniles CRY is driven by light, showing an arrhythmic pattern in DD and cycling under LD. These results, as well as the completely different period length found in the brain circadian oscillations of 2nd post-embryonic stage and juvenile animals, suggest important changes in the properties of the crayfish pacemaker through the development. Therefore these data support a previous idea about the functionality of the circadian system from hatching. (C) 2007 Elsevier Inc. All rights reserved. en_US
dc.language.iso en en_US
dc.title daily and circadian expression of cryptochrome during the ontogeny of crayfish en_US
dc.type Article en_US
dc.identifier.idprometeo 744
dc.identifier.doi 10.1016/j.cbpa.2007.02.014
dc.source.novolpages 151(3):461-470
dc.subject.wos Biochemistry & Molecular Biology
dc.subject.wos Physiology
dc.subject.wos Zoology
dc.description.index WoS: SCI, SSCI o AHCI
dc.subject.keywords Development
dc.subject.keywords Procambarus clarkii
dc.subject.keywords Cryptochromes
dc.subject.keywords Circadian rhythms
dc.subject.keywords Entrainment
dc.relation.journal Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology

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