In general, according to previous studies, pioneer species do not require arbuscular mycorrhizal fungi (AMF) to increase their growth and survival in tropical systems. The aim of this study was to determine the dependence response to AMF of Heliocarpus appendiculatus, a pioneer species, at different phosphorus (P) levels. In a greenhouse experiment, H. appendiculatus seedlings were grown in pots with a sterile vermiculite-sand mixture (1:1). Two sets of pots were set up: One set was inoculated (150 spores per pot) with indigenous AMF from a tropical rain forest at "Los Tuxtlas" (Veracruz, Mexico)
the other set was not inoculated. To each set, 0, 0.02, 0.2, and 2 g L-1 P was added. All pots were watered with 250 mL of nutrient solution. Mycorrhizal plants showed a higher total dry weight and relative growth rate in 0.02 g L-1 P concentration, while nonmycorrhizal plants responded positively at 0.2 g L-1 P
a decrease in plant responses at higher P levels was observed in both treatments. H. appendiculatus showed to have higher relative dependence at lower P concentration (approximate to50%). As levels of P increased, mycorrhizal colonization decreased. Successful growth of pioneer species during succession process may be improved if there is AMF content in soils, prior to disturbance.