dc.contributor.author |
Jaramillo, RD |
|
dc.contributor.author |
Barraza, BC |
|
dc.contributor.author |
Polo, A |
|
dc.contributor.author |
Sara, M |
|
dc.contributor.author |
Contreras, M |
|
dc.contributor.author |
Escamilla, JE |
|
dc.date.accessioned |
2011-01-22T10:26:49Z |
|
dc.date.available |
2011-01-22T10:26:49Z |
|
dc.date.issued |
2002 |
|
dc.identifier.issn |
0008-4166 |
|
dc.identifier.uri |
http://hdl.handle.net/11154/2500 |
|
dc.description.abstract |
The respiratory system of the fastidious beta-proteobacterium Eikenella corrodens grown with limited oxygen was studied. Membranes showed the highest oxidase activity with ascorbate plus N, N, N', N'-tetramethyl-p-phenylenediamine (TMPD) or succinate and the lowest activity with NADH and formate. The presence of a bc(1)-type complex was suggested by the inhibition exerted by 2-heptyl-4-hydroxyquinoline-N-oxide (HOQNO), myxothiazol, and antimycin A on respiration with succinate and by the effect of the latter two inhibitors on the succinate-reduced difference spectra. Respiration with succinate or ascorbate-TMPD was abolished by low KCN concentrations, suggesting the presence of a KCN-sensitive terminal oxidase. Cytochromes b and c were spectroscopically detected after reduction with physiological or artificial electron donors, whereas type a and d cytochromes were not detected. The CO difference spectrum of membranes reduced by dithionite and its photodissociation spectrum (77 K) suggested the presence of a single CO compound that had the spectral features of a cytochrome o-like pigment. High-pressure liquid chromatography analysis of membrane haems confirmed the presence of haem B |
en_US |
dc.description.abstract |
in contrast, haems A and O were not detected. Peroxidase staining of membrane type c cytochromes using SDS-PAGE revealed the presence of five bands with apparent molecular masses of 44, 33, 30, 26, and 14 kDa. Based on our results, a tentative scheme of the respiratory chain in E. corrodens, comprising (i) dehydrogenases for succinate, NADH, and formate, (ii) a ubiquinone, (iii) a cytochrome bc(1), and (iv) a type-cbb' cytochrome c oxidase, is proposed. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
The aerobic electron transport system of Eikenella corrodens |
en_US |
dc.type |
Article |
en_US |
dc.identifier.idprometeo |
2093 |
|
dc.identifier.doi |
10.1139/W02-084 |
|
dc.source.novolpages |
48(10):895-902 |
|
dc.subject.wos |
Biochemistry & Molecular Biology |
|
dc.subject.wos |
Biotechnology & Applied Microbiology |
|
dc.subject.wos |
Immunology |
|
dc.subject.wos |
Microbiology |
|
dc.description.index |
WoS: SCI, SSCI o AHCI |
|
dc.subject.keywords |
Eikenella corrodens |
|
dc.subject.keywords |
respiratory chain |
|
dc.subject.keywords |
bc(1) complex |
|
dc.subject.keywords |
oxidase cbb |
|
dc.relation.journal |
Canadian Journal of Microbiology |
|