dc.contributor.author |
De Lara, LV |
|
dc.contributor.author |
Becerril, C |
|
dc.contributor.author |
Montano, M |
|
dc.contributor.author |
Ramos, C |
|
dc.contributor.author |
Maldonado, V |
|
dc.contributor.author |
Melendez, J |
|
dc.contributor.author |
Phelps, DS |
|
dc.contributor.author |
Selman, M |
|
dc.contributor.author |
Pardo-Cemo, Annie |
|
dc.date.accessioned |
2011-01-22T10:28:12Z |
|
dc.date.available |
2011-01-22T10:28:12Z |
|
dc.date.issued |
2000 |
|
dc.identifier.issn |
10400605 |
|
dc.identifier.uri |
http://hdl.handle.net/11154/2580 |
|
dc.description.abstract |
During lung injury, fibroblasts migrate into the alveolar spaces where they can be exposed to pulmonary surfactant. We examined the effects of Survanta and surfactant protein A (SP-A) on fibroblast growth and apoptosis and on type I collagen, collagenase-1, and tissue inhibitor of metalloproteinase (TIMP)-1 expression. Lung fibroblasts were treated with 100, 500, and 1,000 mg/ml of Survanta |
en_US |
dc.description.abstract |
10, 50, and 100 mg/ml of SP-A |
en_US |
dc.description.abstract |
and 500 mg/ml of Survanta plus 50 mg/ml of SP-A. Growth rate was evaluated by a formazan-based chromogenic assay, apoptosis was evaluated by DNA end labeling and ELISA, and collagen, collagenase-1, and TIMP-1 were evaluated by Northern blotting. Survanta provoked fibroblast apoptosis, induced collagenase-1 expression, and decreased type I collagen affecting mRNA stability similar to 10-fold as assessed with the use of actinomycin D. Collagen synthesis and collagenase activity paralleled the gene expression results. SP-A increased collagen expression similar to2-fold and had no effect on collagenase-1, TIMP-1, or growth rate. When fibroblasts were exposed to a combination of Survanta plus SP-A, the effects of Survanta were partially reversed. These findings suggest that surfactant lipids may protect against intraluminal fibrogenesis by inducing fibroblast apoptosis and decreasing collagen accumulation. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
Surfactant components modulate fibroblast apoptosis and type I collagen and collagenase-1 expression |
|
dc.type |
Artículo de investigación |
en_US |
dc.identifier.idprometeo |
2445 |
|
dc.source.novolpages |
279(5) |
|
dc.subject.wos |
Physiology |
|
dc.subject.wos |
Respiratory System |
|
dc.description.index |
WoS: SCI, SSCI o AHCI |
|
dc.subject.keywords |
pulmonary fibrosis |
|
dc.subject.keywords |
surfactant protein A |
|
dc.relation.journal |
American Journal of Physiology-Lung Cellular and Molecular Physiology |
|
dc.relation.journal |
American Journal of Physiology-Lung Cellular and Molecular Physiology |
|
dc.description.Departamento |
Departamento de Biología Comparada |
|