dc.contributor.author |
Selman, M |
|
dc.contributor.author |
Ridge, K |
|
dc.contributor.author |
BarRíos, R |
|
dc.contributor.author |
Sznajder, JI |
|
dc.contributor.author |
Pardo-Cemo, Annie |
|
dc.date.accessioned |
2011-01-22T10:28:17Z |
|
dc.date.available |
2011-01-22T10:28:17Z |
|
dc.date.issued |
1996 |
|
dc.identifier.issn |
1073449X |
|
dc.identifier.uri |
http://hdl.handle.net/11154/2928 |
|
dc.description.abstract |
Exposure of adult rats to 100% O-2 produces a lethal injury by 72 h. We reasoned that matrix metalloproteinases participate in the pathogenesis of hyperoxic lung injury. To that end we studied the expression and activity of gelatinases A and B and interstitial collagenase in lung tissues and bronchoalveolar ravage fluids (BALF) of rats exposed to 100% oxygen for 60 h. Gelatin zymography of BALF samples revealed a similar to 72 kDa molecular species both in controls and oxygen-exposed animals. In addition, BALF from hyperoxic rats exhibited a 95-kDa gelatinase. Likewise, BALF total gelatinolytic and collagenolytic activities were significantly increased in oxygen-exposed rats. In situ hybridization revealed an increase in type IV collagenases as well as interstitial collagenase mRNAs in the oxygen-exposed lungs. The three enzymes were expressed by alveolar macrophages, and in variable degrees by interstitial and alveolar epithelial cells. Immunoreactive gelatinase B and collagenase paralleled the cell localization of the mRNAs but were also detected in the alveolar walls and interstitium. In situ zymography showed gelatinolytic activity in frozen sections of oxygen-exposed lungs but not in normal lungs. The upregulation of these metalloproteinases during acute exposure to 100% O-2 suggests that they might contribute to hyperoxic lung damage through the degradation of extracellular matrix components. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
Increased expression of gelatinases and collagenase in rat lungs exposed to 100% oxygen |
|
dc.type |
Artículo de investigación |
en_US |
dc.identifier.idprometeo |
3049 |
|
dc.source.novolpages |
154(4):1067-1075 |
|
dc.subject.wos |
Critical Care Medicine |
|
dc.subject.wos |
Respiratory System |
|
dc.description.index |
WoS: SCI, SSCI o AHCI |
|
dc.relation.journal |
American Journal of Respiratory and Critical Care Medicine |
|
dc.description.Departamento |
Departamento de Biología Comparada |
|