Analysis of different cell death processes of prepubertal rat oocytes in vitro

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Show simple item record 2011-01-22T10:25:44Z 2011-01-22T10:25:44Z 2010
dc.identifier.issn 13608185
dc.description.abstract The processes of cell death were studied in vitro in populations of oocytes isolated from prepubertal rats. In order to identify apoptosis, the externalized phosphatidylserine was recognized with Annexin-V coupled to FITC and the fragmentation of DNA was demonstrated by means of electrophoresis. Oocytes were tested for autophagy by means of the incorporation of monodansylcadaverine and monitoring Lc3-I/Lc3-II by western blot. The expression of mRNA marker genes of autophagy and of apoptosis was studied by means of RT-PCR in pure populations of oocytes. Some oocytes expressed at least one of the following markers: caspase-3, lamp1 and Lc3. Some oocytes were positive to Annexin-V or to monodansylcadaverine. However, most of them were simultaneously positive to both markers. The relative frequency of oocytes simultaneously positive to markers of apoptosis and autophagy did not change in the different ages studied. The transformation of Lc3-I in Lc3-II was present in all populations of oocytes studied. The mRNAs for caspase-3, lamp1 and Lc3 were present in all populations of oocytes analyzed. Our results demonstrate that oocytes of rats from new born to prepubertal age are eliminated by means of three different cell death processes: apoptosis, autophagy and a mixed event in which both routes to cell death participate in the same cell. en_US
dc.language.iso en en_US
dc.title Analysis of different cell death processes of prepubertal rat oocytes in vitro
dc.type Artículo de investigación en_US
dc.identifier.idprometeo 251
dc.identifier.doi 10.1007/s10495-009-0448-1
dc.source.novolpages 15(4):511-526
dc.subject.wos Biochemistry & Molecular Biology
dc.subject.wos Cell Biology
dc.description.index WoS: SCI, SSCI o AHCI
dc.subject.keywords Apoptosis
dc.subject.keywords Autophagy
dc.subject.keywords Annexin-V
dc.subject.keywords DNA-fragmentation
dc.subject.keywords Lc3
dc.subject.keywords Monodansylcadaverine
dc.relation.journal Apoptosis
dc.description.Departamento Departamento de Biología Comparada

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