dc.contributor.author |
Montano, M |
|
dc.contributor.author |
Selman, M |
|
dc.contributor.author |
Ramirez, R |
|
dc.contributor.author |
Ramos, C |
|
dc.contributor.author |
Pardo-Cemo, Annie |
|
dc.date.accessioned |
2011-01-22T10:28:42Z |
|
dc.date.available |
2011-01-22T10:28:42Z |
|
dc.date.issued |
1992 |
|
dc.identifier.issn |
3008207 |
|
dc.identifier.uri |
http://hdl.handle.net/11154/3484 |
|
dc.description.abstract |
The levels of collagenase inhibitor, both free and bound to metalloproteinases, were evaluated at 7 days [deposit phase (DP)] and 14 days [resorptive phase (RP)] of evolution of the subcutaneous carrageenin-induced granuloma in the guinea pig. The level of free collagenase inhibitor was considerably higher in the supernatant of DP granulomas (7.95 +/- 1.53 U/mg protein) as compared to that of RP granulomas (2.53 +/- 0.41 U/mg protein). When the samples were heated at acid pH to release the inhibitor from metalloproteinase-inhibitor complexes, free inhibitor was recovered in both phases. However, the units of recovered collagenase inhibitor were several fold higher in all RP granulomas in comparison with DP granulomas (6.88 +/- 2.46 vs 1.5 +/- 0.53). Therefore, DP and RP tissues exhibited similar total amount of tissue inhibitor. By HPLC, collagenase inhibitor activity was localized in a fraction consistent with the size of TIMP. These results suggest a different balance of collagenase and collagenase inhibitor during the evolution of the granuloma |
en_US |
dc.description.abstract |
an excess of inhibitor over metalloproteinases appears to predominate during the phase of collagen accumulation contrasting with an inverse situation when the granuloma is healing. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
Collagenase-Inhibitory Activity in Deposit and Resorption Phases of Guinea-Pig Carrageenan Granuloma |
|
dc.type |
Artículo de investigación |
en_US |
dc.identifier.idprometeo |
3363 |
|
dc.source.novolpages |
28(4):307-315 |
|
dc.subject.wos |
Cell Biology |
|
dc.subject.wos |
Orthopedics |
|
dc.description.index |
WoS: SCI, SSCI o AHCI |
|
dc.subject.keywords |
Carrageenan-Granuloma |
|
dc.subject.keywords |
Collagenase |
|
dc.subject.keywords |
Metalloproteinases |
|
dc.subject.keywords |
TIMP |
|
dc.relation.journal |
Connective Tissue Research |
|
dc.description.Departamento |
Departamento de Biología Comparada |
|