dc.contributor.author | Martos, MA | |
dc.contributor.author | Vazquez, FM | |
dc.contributor.author | Benassi, FO | |
dc.contributor.author | Hours, RA | |
dc.date.accessioned | 2011-01-22T10:25:50Z | |
dc.date.available | 2011-01-22T10:25:50Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1516-8913 | |
dc.identifier.uri | http://hdl.handle.net/11154/13982796 | |
dc.description.abstract | Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A. niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be suitable to be used in food processing in order to reduce the production of methanol. | en_US |
dc.language.iso | en | en_US |
dc.title | Production of Pectinases by A-niger: Influence of Fermentation Conditions | en_US |
dc.type | Article | en_US |
dc.identifier.idprometeo | 468 | |
dc.source.novolpages | 52(3):567-572 | |
dc.subject.wos | Biology | |
dc.description.index | WoS: SCI, SSCI o AHCI | |
dc.subject.keywords | Pectinases | |
dc.subject.keywords | Polygalacturonase | |
dc.subject.keywords | Pectinesterase | |
dc.subject.keywords | Aspergillus niger | |
dc.subject.keywords | Response surface methodology | |
dc.subject.keywords | Citrus pectin | |
dc.relation.journal | Brazilian Archives of Biology and Technology |
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