Cytotoxicity of the b-carboline alkaloids harmine and harmaline in human cell assays in vitro

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Show simple item record Jimenez, J Riveron-Negrete, L Abdullaev, F Espinosa-Aguirre, J Rodríguez-Arnaiz, R 2011-01-22T10:26:08Z 2011-01-22T10:26:08Z 2008
dc.identifier.issn 9402993
dc.description.abstract beta-Carboline alkaloids are natural products widely distributed in plants and also found in alcoholic beverages, well-cooked foods and tobacco smoke. Various authors have reported genotoxic activities of several carboline in prokaryotic and eukaryotic cells that have been attributed to their abilities to intercalate into DNA. But studies on the genotoxic and on the cytotoxic potencies in human cells in vitro are not found in the literature. In the present study the toxicities of one full aromatic beta-carboline alkaloid (harmine) and one dihydro-p-carboline alkaloid (harmaline) were evaluated by means of two in vitro human cell assays: the cytoclialasin-B blocked micronucleus (CBMN) assay and the viability/colony formation assay with four different human cultured non-transformed (CCD18Lu) and transformed (HeLa, C33A and SW480) cells. Neither alkaloid was able to induce micronuclei levels above that of control levels in a wide range of doses tested en_US
dc.description.abstract although, harmine at the highest concentrations assayed induced apoptotic as well as necrotic cells. Harmine produced a good viability of all cell lines assayed (control and tumor) while harmaline significantly reduced the viability of transformed and non-transformed cell lines in a dose-dependent manner. Harmine displayed a dose-dependent inhibitory effect on cell proliferation against all human carcinoma cells, but the SW480 transformed cell line showed a higher sensitivity. These results suggested that harmine was identified as a useful inhibitor of tumor development. (C) 2008 Elsevier GmbH. All rights reserved. en_US
dc.language.iso en en_US
dc.title Cytotoxicity of the b-carboline alkaloids harmine and harmaline in human cell assays in vitro
dc.title Inglés
dc.title Inglés
dc.type Artículo de investigación en_US
dc.identifier.idprometeo 815
dc.identifier.doi 10.1016/j.etp.2007.12.003
dc.source.novolpages 60(40638):381-389
dc.subject.wos Pathology
dc.subject.wos Toxicology
dc.description.index WoS: SCI, SSCI o AHCI
dc.subject.keywords beta-carboline alkaloids
dc.subject.keywords harmine
dc.subject.keywords harmaline
dc.subject.keywords cytochalasin-B blocked micronucleus (CBMN) assay
dc.subject.keywords viability and cell colony formation assay
dc.relation.journal Experimental and Toxicologic Pathology
dc.description.Departamento Departamento de Biología Comparada

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